Meldonium, or mildronate

Meldonium [3-(2,2,2-trimethylhydraziniumyl)propionate; mildronate] was added to the World Anti-Doping Agency (WADA) list of prohibited substances effective 1 January 2016. It was originally developed in Latvia in the late 1970s as a growth-promoting agent for cattle and poultry (1). Later, once all the mechanisms of action of the substance were established, the main indication of meldonium has been to protect  the heart in ischemic states, such as myocardial infarct. Meldonium has also been used in the treatment of diabetes and cerebral infarct in order to protect nervous tissue (2,3). Meldonium is used clinically primarily in Eastern European countries. It has been available in pharmacies without a prescription. Meldonium has not been granted market approval in Finland, Western Europe or the United States. Meldonium is administered as capsules or intravenous injections.

The chemical structure of meldonium resembles that of L-carnitine. Its efficacy is mainly based on the fact that it inhibits carnitine synthesis and its effects in several mechanisms while also promoting the removal of carnitine through the kidneys (4). Carnitine is an amino acid which is biosynthesized as well as obtained from food. It plays an important role in the oxidation of fatty acids in cells. When tissue suffers from lack of oxygen, meldonium acts to inhibit the fatty acid oxidating effect of carnitine so that the energy balance in, say, a heart cell shifts in the direction of carbohydrate oxidation. As a consequence, the generation of high-energy adenosine triphosphate (ATP)—which is needed in muscle contraction—requires less oxygen and, therefore, the heart functions with greater efficiency (2). It also reduces the amount of intermediate products that are generated in a low-oxygen environment and are harmful to the cell. Meldonium also stimulates blood circulation in many organs of the body by increasing the nitric oxide concentration (5).

In clinical use, only minor side effects have been recorded. However, there are few studies on the effects of overdoses. The most common side effects include headache, nervousness, dizziness, sleeplessness, allergic symptoms, a fall in blood pressure and tachycardia.

There is relatively little scientific evidence of meldonium being able to enhance athletic performance. However, it has been established that meldonium demonstrates an increase in endurance performance and physical working capacity, as well as improved rehabilitation after exercise (6,7). Meldonium also stimulates the central nervous system, which may have a beneficial effect on athletic performance (8). On the whole, meldonium has a wide range of effects, which, combined with easy availability and effective marketing, explains why it has been used in many different disciplines. In 2015—when meldonium had not yet been banned—WADA tested 8,320 doping samples for meldonium (8). Of these samples, 182 (2.2 per cent) tested positive for meldonium. Most of the positive samples came from athletes in sports that require muscular strength. However, meldonium use was also discovered in endurance sports.

Meldonium leaves the body relatively quickly through the kidneys, where it is secreted into urine mainly as such. Depending on the dose and period of use, the levels of meldonium in blood plasma are halved every 6–14 hours (9). In practice, meldonium will show up in doping tests for 2–4 days after the athlete stops taking it.

In WADA’s list of prohibited substances and methods, meldonium is listed under S4.5 ‘Metabolic modulators’. These substances are prohibited at all times. They are not ‘specified substances’, and their use normally carries a sanction of ineligibility of four (4) years.

Timo Seppälä
Finnish Center for Integrity in Sports (FINCIS)
Finnish Antidoping Agency (FINADA)
Medical Director


1. Eremeev A, Kalvinsh, V. Semenikhina ym.: 3-(2,2,2-Trimethylhydrazinium) propionate and method for the preparation and use thereof //. US Patent No. 4481218 [06 November 1984].

2. Dambrova, M, Liepinsh, E, Kalvinsh, I: Mildronate. Cardioprotective action through carnitine-lowering effect. Trends Cardiovasc. Med. (2002) 12: 275–279.

3. Sjakste N, Gutcaits A, Kalvinsh I: Mildronate: An Antiischemic Drug for Neurological Indications. CNS Drug Reviews (2005) 11: 151–168.

4. Dambrova M., Makrecka-Kuka M, Vilskersts R ym.: Pharmacological effects of meldonium: Biochemical mechanisms and biomarkers of cardiometabolic activity, Pharmacol Res (2016), (in press).

5. Sjakste N, Kleschyovc AL, BoucherJ-L ym.: Endothelium- and nitric oxide-dependent vasorelaxing activities of gamma-butyrobetaine esters: possible link to the antiischemic activities of mildronate. Eur J Pharmacol (2004) 495: 67–73.

6. Kakhabrishvili Z, Chabashvili N, Akhalkatsi V ym: Mildronate effect on physical working capacity among highly qualified judokas. Ann. Biomed. Res. Edu. (2002) 2: 551.

7. Dzintare M, Kalvins I: Mildronate increases aerobic capabilities of athletes through carnitine-lowering effect. Curr. Issues New Ideas Sport Sci. (2012) 5: 59.

8. Görgens C, Guddat S, Dib J ym: Mildronate (Meldonium) in professional sports –monitoring doping control urine samples using hydrophilic interaction liquid chromatography – high resolution/high accuracy mass spectrometry Drug Test. Analysis (2015) 7: 973–979.

9. Zhang J, Cai L-J, Yang J ym.: Nonlinear pharmacokinetic properties of mildronate capsules: a randomized, open-label, single- and multiple-dose study in healthy volunteers. Fundamental Clin Pharmacol (2013) 27: 120–128.